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Inserts encoding the neoAgs had been obtained from Genscript and cloned into MSCV-IRES-Thy1.1 DEST and pBK-T-SFV-b12A using BglII&SalI and Apa I sites, respectively. MSCV-IRES-Thy1.1 DEST was a present from Anjana Rao (Addgene plasmid # 17442). Plasmids pMSCV-TMGP69-IRES-Thy1.1, pMSCV-TMGP73-IRES-Thy1.1 encoding the neoAgs and management plasmid (MSCV-IRES-Thy1.1 DEST) had been used to prepare retrovirus for transduction experiments. A needle tumor biopsy was extracted at diagnosis and preserved at -80°C in RNAlater.
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However, within the second group all peptides contained the mutation at non-anchor residues. Indeed, it has been recently shown the relevance of the place of the mutated amino acid in neoAg choice . HLA class I molecules expressed by sufferers had been identified utilizing sequencing information and all potential peptides containing mutated residues were cam69 chosen for prediction of peptide binding to HLA. By utilizing a threshold of 500 nM in affinity and a % Rank Table 1, Supplementary Table S4). NeoAg-specific responses have been evaluated at day 21 of co-culture by utilizing a human IFN-γ ELISPOT set (BD-Biosciences) following manufacturer’s instructions.
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Thus, new therapeutic methods are mandatory in TNBC patients. For TMB calculation, SNVs detected by Mutect2 (3.8) have been used. For neoantigen selection, SNVs known as by 2 or extra callers were considered. TMB was calculated because the ratio between the variety of mutations and the size of the sequenced areas (35.7 Mb). For those sufferers with available RNAseq, quartiles of FPKM have been calculated.
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